Ion exchange chromatography lab manual

Ion exchange chromatography using a cation exchanger. 1. Equilibration 2. Sample application and wash 3. Elution 4. Regeneration Type of purification Manual or system purification System purification Format Bottles of chromatography resins Small-column cartridges (1 and 5 mL) Other columns Format name Lab pack HiTrap™ HiScreen™, HiPrep. Ion exchange chromatography: Ion-exchange chromatography is a versatile, high resolution chromatography techniques to purify the protein from a complex mixture. In addition, this chromatography has a high loading capacity to handle large sample. Ion exchange chromatography (IEX) separates biomolecules according to differences in their net surface charge. Property Technique Charge Ion exchange chromatography (IEX) Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC).

B. Preparation of Ion Exchange Columns The purpose of this part of the experiment is to prepare small columns of a weak anion exchange resin called DEAE (diethylaminoethyl) Cellulose and a weak cation exchange resin called CM (carboxymethyl) Cellulose. These resins are listed in Table of the lab manual. Principles of ion exchange This chapter provides a general introduction to the theoretical principles that underlie every ion exchange separation. An understanding of these principles will enable the separation power of ion exchange chromatography (IEX) to be fully appreciated. Practical aspects of performing a separation are covered in Chapter 2. I. Ion Exchange Chromatography 1. Add ml Equilibration Buffer to the Protein Extract vial. Periodically vortex the tube until the protein completely dissolves. 2. Centrifuge the tu be for 2 minutes at maximum speed in a microcentrifuge to remove the froth. 3. Clamp the Cationic Chromatography Column in an upright position to the stand. 4.

30 thg 3, include the expanding range of Capto chromatography media for lab- and New Selection Guide: Ion Exchange Chromatography Columns and. Preparation of DEAE-cellulose column. 7. Separation of proteins by Ion Exchange Chromatography. 8. Determination of molecular weight of protein using SDS-. 26 thg 6, Ion Exchange Chromatography (IEC) is a powerful liquid chromatographic technique used for bioseparation. The separation is done by a.